‘Research casts doubt on theory of cause of chronic fatigue’: Chicago Tribune, 17 March 2011

March 20, 2011

From the Chicago Tribune, 17 March 2011 (story by Trine Tsouderos).

A high-profile scientific paper that gave enormous hope to patients diagnosed with chronic fatigue syndrome, and even prompted some to begin taking potent anti-HIV drugs, has been largely discredited by subsequent research.

Evidence is mounting that a retrovirus called XMRV is not a new human pathogen infecting millions, as was feared, but a laboratory contaminant.

Cancer biologist Robert Silverman, a key researcher at Cleveland Clinic's Lerner Research Institute who worked on studies that linked XMRV to chronic fatigue syndrome and prostate cancer, told the Tribune his lab had stored a cell line known to harbor XMRV and he was working to determine if contamination occurred. Virologists who have examined work by Silverman and others have raised serious questions about contamination, an unfortunate but not unusual mishap in the field.

“I am concerned about lab contamination, despite our best efforts to avoid it,” Silverman wrote in an e-mail, adding that similar cell lines “are in many, many labs around the world. Contamination could come from any one of a number of different sites.”

A European research team this week reported being unable to find any evidence of XMRV in the blood of people diagnosed with chronic fatigue syndrome and their healthy peers, the latest in a stream of studies in which researchers looking for the retrovirus in the blood of both sick and healthy people have come up empty. Others have reported no evidence of the retrovirus in the blood of patients who were previously found to be XMRV-positive.

The Tribune reported last year that the original research on chronic fatigue syndrome and XMRV had led some patients to get tested for the retrovirus and take anti-retroviral drugs intended to treat HIV, which causes AIDS. The situation highlights the danger in putting too much stock in one scientific study, even one in a prestigious journal. Studies need to be replicated, and early research is often proved wrong.

The original study, published in Science in 2009, was led by retroviral immunologist Judy Mikovits of the private Whittemore Peterson Institute for Neuro-Immune Disease in Reno, Nev. The institute plans to open a clinic that in May would begin treating patients diagnosed with chronic fatigue syndrome and other neuro-immune diseases. Despite the newer research, its leaders strongly deny that contamination could account for their findings.

“It is clearly a human infection,” Mikovits, the institute's director of research, told an audience at a January presentation hosted by a California alternative medical practice. “It is clearly circulating through the population as is our fear and your fear.”

Scientists say there is no evidence to support her statement.

“Saying that is just inciting fear,” said Columbia University virologist Vincent Racaniello.

Mikovits, who once worked at the National Cancer Institute in Frederick, Md., has made increasingly broad statements about XMRV. At the January talk, she showed a slide connecting XMRV to a list of frustrating medical conditions like ALS, Parkinson's disease, multiple sclerosis and dementia. She also linked it to autism. But no published data exist to support those links.

Mikovits also talked about potential treatments, including the powerful anti-retroviral drugs used to treat people who have HIV. These have not been proved safe or effective for people with chronic fatigue syndrome or any of the other conditions listed.

The WPI's director of clinical services, Dr. Jamie Deckoff-Jones, who has chronic fatigue syndrome and has taken anti-retroviral drugs for a year, is using a personal blog to allege a cover-up by researchers seeking to discredit the XMRV link.

“So is there motivation for the cover-up and baseless attacks against Dr. Mikovits?” she wrote in a posting that has been widely circulated on patient forums. “They cannot attack the data because it is impeccable.”

WPI President Annette Whittemore, whose daughter has been diagnosed with chronic fatigue syndrome, said in an interview that she thinks politics are at play.

“I thought we were going to solve my daughter's illness or at least fund more significant treatments,” said Whittemore, who founded the institute. “I didn't think we would have such political pushback. That was so naive of me.”

Whittemore also defended patients trying anti-retrovirals, saying they are safe if used under an experienced doctor's care.

“Patients choose to try these drugs because they are so sick they have lost their entire lives to this illness,” she wrote in an e-mail. “As far as I am concerned, they are the pioneers paving the way forward for other sufferers.”

In her presentation, Mikovits also described the antiretroviral drugs as “very well tolerated” by patients trying them for chronic fatigue syndrome.

“Very clearly something is going on in the majority of people being treated,” she said. “Most don't notice they are taking them.”

Physicians who work with HIV patients say antiretroviral drugs can cause significant side effects and that efficacy cannot be determined through anecdotes.

The chasm between the WPI and its supporters and many in the scientific community is emblematic of a new, modern-day dynamic in which patients keep close tabs on the work of researchers and feel empowered to challenge that work and form strong opinions about the quality of it.

Early on, many in the online chronic fatigue community threw their support behind WPI, believing strongly that XMRV was the cause of their illness. More than 1,000 people have paid for non-FDA-approved XMRV blood tests from a commercial lab associated with WPI and headed by Whittemore's husband, Harvey, according to state records. The tests range from $249 to $450, according to the lab website.

Patients' ardent support for XMRV as a cause has continued as other research teams have failed to find any evidence that it was true.

On one patient message board, a commenter wrote in February about not only having contributed multiple times to WPI but also having “sent letters, e-mails, tried to contribute ideas, talked with both Judy M. and Annette, considered whether there's an opportunity for venture capital funding, am willing to protest, knit a pair of socks, etc.”

This month, 4,000 scientists and clinicians gathered in Boston for a retroviral conference that included 10 presentations offering evidence that XMRV is a lab contaminant. Mikovits did not attend.

Retrovirologist Jonathan Stoye, who co-wrote a supportive commentary that accompanied Mikovits' original study linking chronic fatigue syndrome and XMRV, said he has since changed his mind.

“I think there are serious problems,” said Stoye, who works at MRC National Institute for Medical Research in London.

His co-author, John Coffin, a retrovirologist at Tufts University, agreed the evidence for a link between XMRV and human disease had been seriously weakened.

“I think most people are reasonably convinced that there is not much left anymore,” Coffin said. But, he said, “I don't think everything has been nailed down.”

Coffin began harboring doubts about Mikovits' study as negative evidence piled up and after he, researcher Vinay Pathak at the National Cancer Institute and their colleagues found what they believe to be the parent viruses of XMRV.

The viruses, according to research Pathak presented at the Boston conference, recombined in a cell line called 22RV1 to create a new retrovirus — XMRV — sometime in the 1990s. The work is in the publication process.

That widely used cell line had been stored in Silverman's lab before he found evidence of the retrovirus in the prostate tissue of patients with a form of prostate cancer.

“22RV1 cells were once previously (more than a year earlier) grown in my lab but were being stored in a liquid nitrogen freezer at the time, and not the same freezer used to store prostate tissues,” Silverman wrote in an e-mail. “At the time it was unknown that 22RV1 cells were infected with XMRV.”

In the field of virology, contamination has sometimes been mistaken for real results. Greg Towers, a virologist from University College London, notes that the technology used is so sensitive that it takes only one molecule of genetic material to contaminate a sample.

Scientists have been reluctant to shut the door completely on the possibility that XMRV really is tied to human disease. Some questions remain unanswered, said Racaniello, of Columbia University. “I don't think it is time to put a lid on it,” he said. “You have to carry the whole thing to its conclusion.”

The XMRV story is, to Racaniello, an amazing opportunity for people to watch how science works in real time.

“It is like Watergate,” he said. “You saw the Constitution work. You think, oh my gosh, it works! And this is science working. Science determines the truth. … It always sorts it out in the end.”


Chicago Tribune News Blog, 18 March 2011

10 thoughts on “‘Research casts doubt on theory of cause of chronic fatigue’: Chicago Tribune, 17 March 2011”

  1. This article is tosh. She’s a food writer. If you are going to post this, how about posting articles from those who actually know the subject, like Hilary Johnson or Mindy Kitei?

    There is no evidence of contamination. Silverman is not anything but happy with his research. He detected XMRV in a way that could not have been from contamination. Mikovits has never said people should take ARV’s, she has only talked about what would happen if it is causing disease.

    The reason they cannot detect XMRV in the earlier generations of the cell line, is because their assay is not sensitive enough, and they used testosterone on the later generations which would have increased the level of virus to a detectable level. Also, they do not know if the mice they think they found the PreXMRV in were used in any generation.

    So what has happened? The cell line, which has come from a patient infected with XMRV, has contaminated the mice.

  2. It was Mikovits that started the renewed vigour and interest in the viral link to disease into ME. It has given the area a wake up call and patients hope. More people know of our plight now despite the UK government’s intention to paly it down for economic purposes with their agenda highlighted by the DWP’s involvement in the PACE trial-the first scientific study that the DWP have ever funded. Fact obtained in a FOI request but that I knew about all along.

    I just hope that if the consensus over XMRV proves that the link is unproven, then a directive to an agreement over diagnostic criterias, funding for research into genuine ME and the drive for a definitive test will be the focus. A long-term drive with funds outweighing those for other illnesses is needed now to address the imbalance shown for a disease neglected for decades.

  3. Sorry, that comment was intended for the Amy Marcus article that was in the Wall St Journal.

  4. Please do not allow a “Food Writer” to sow doubt, where doubt doesn’t exist and on a topic where a food writer is so obviously out of her depth.

    Silverman may have felt the need to do precautionary checks (as any responsible scientist would) but he hasn’t expressed any doubt in the validity of XMRV as a human pathogen.

    Furthermore, the WPI will soon reveal evidence that will blow all of this nonsense into the weeds.

    1. Dio,

      I have heard this comment before. Here and elsewhere.

      What evidence exactly are you expecting from Dr Mikovits may I ask? Are you referring to her speech at the conference in May and/or news of a ‘treatment’?

  5. As one of my highly intelligent friends with ME has pointed out….

    the notion of a “lab-contaminant” virus which can select to contaminate only patient samples and not the control samples in the same experimental run is really a notion which should be mind-bogglingly worrying for all scientists in all fields and could well be the harbringer of the apocalypse….

    or we could be a little bit more sensible, and simply dismiss the thoughts of anybody stupid and ignorant enough to suggests this ludicrous notion in the first place.

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