TGI Friday! Our weekly round-up of recently published research abstracts | 11 July 2014

July 11, 2014


From Clinical and Cellular Immunology, 14 June 2014 (link downloads full paper).

Characterization of Natural Killer Cell Phenotypes in Chronic Fatigue Syndrome/Myalgic Encephalomyelitis

Teilah K. Huth(1,2*), Ekua W. Brenu(1,2), Thao Nguyen(1,2), Sharni L.Hardcastle(1,2), Samantha Johnston(1,2), Sandra Ramos(1,2), Donald R. Staines(2,3) and Sonya M. Marshall-Gradisnik(1,2)

1) National Centre for Neuroimmunology and Emerging Diseases, Griffith Health Institute, Griffith University, Southport, QLD, Australia
2) School of Medical Science, Griffith University, Southport, QLD, Australia
3) Queensland Health, Gold Coast Public Health Unit, Robina, QLD, Australia
*Corresponding author: Teilah Huth, BBioMedSc(Hons), National Centre for Neuroimmunology and Emerging Diseases, Griffith Health Institute, Griffith University, Parklands, QLD 4222, Australia, Tel: +61 7 5678
9283; E-mail: t.huth@griffith.edu.au

Abstract

OBJECTIVE

Natural Killer (NK) cells are classified into different phenotypes according to the expression of the surface markers CD56 and CD16. Each NK cell phenotype has a role in the immune response through cytotoxic activity or cytokine production. Reduced NK cell cytotoxic activity is a consistent finding in patients with Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME) and investigations into the potential causes of reduced NK cell cytotoxic activity have predominantly focused on total NK cells. The purpose of this study was to investigate and characterize four NK cell phenotypes in CFS/ME.

METHODS

Twenty nine CFS/ME patients (mean age ± SEM=48.28 ± 2.63) meeting the 1994 Fukuda definition and 27 healthy controls (mean age ± SEM=49.15 ± 2.51) were included in this study. Flow cytometric protocols identified CD56brightCD16-/dim, CD56dimCD16-, CD56dimCD16+ or CD56-CD16+ NK cells for the measurement of surface markers including adhesion molecules CD2, CD18, CD11a, CD11b and CD11c, natural cytotoxicity receptors, Killer Immunoglobulin Like Receptors, signalling lymphocytic activation molecules and cell maturation (CD57).

Following stimulation, NK cell phenotype expression of CD107a and CD107b was measured as a marker for degranulation. Intracellular staining measured lytic proteins including perforin, Granzyme A and Granzyme B in the four NK cell phenotypes.

RESULTS

In the CFS/ME group, CD56brightCD16-/dim NK cell co-expression of adhesion molecules CD2 and CD18 was significantly reduced. Granzyme B was significantly decreased in CD56dimCD16+ and CD56-CD16+ NK cells from CFS/ME patients. CD57 expression on CD56dimCD16+ NK cells from CFS/ME patients was significantly increased.

CONCLUSION

This is the first study to characterize four NK cell phenotypes in CFS/ME by investigating surface and intracellular molecules necessary for NK cell effector function. The data suggests that a combination of impairments in CD56dimCD16+ NK cells from CFS/ME patients may contribute to reduced cytotoxic activity of this
phenotype.


1 thought on “TGI Friday! Our weekly round-up of recently published research abstracts | 11 July 2014”

  1. Relevant immune system markers in CFS/ME patients were, as quoted directly from the research text above:
    “.. SIGNIFICANTLY reduced, SIGNIFICANTLY decreased and SIGNIFICANTLY increased ..”
    (full details above).

    This is not the first study of this kind showing these kinds of immune system dysfunction.

    It would be encouraging if these kinds of impressive findings were generally known and SIGNIFICANTLY acted upon by NICE, the practising medical, nursing & social work communities and significantly expanded upon by other scientists.

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